Journal: The Journal of Clinical Investigation

Article Title: Absence of the intracellular lipolytic inhibitor G0S2 enhances intravascular triglyceride clearance and abolishes diet-induced hypertriglyceridemia

doi: 10.1172/JCI181754

Figure Lengend Snippet: ( A ) Mouse model building scheme. Seven-week-old male WT and G0s2 –/– mice were fed a Western diet and treated with either control or Ldlr-ASO weekly for 12 weeks. ( B ) Western blot analysis of mouse liver for LDLR and G0S2. ( C and D ) Fasting plasma levels of TG ( C ) and total cholesterol, free cholesterol, and cholesterol ester ( D ) after diet treatment ( n = 10). Chol., cholesterol. ( E and F ) FPLC analysis of pooled plasma TG ( E ) and total cholesterol ( F ) ( n = 10), with AUC quantification (insets). ( G ) Fasting plasma free FA (FFA) levels ( n = 7). Data represent mean ± SEM. ** P < 0.01, **** P < 0.0001 by 1-way ( C and G ) or 2-way ANOVA ( D ).

Article Snippet: Plasma cholesterol levels were measured using the Amplex Red Cholesterol Assay Kit (Thermo Fisher Scientific, A12216), according to the manufacturer’s instructions.

Techniques: Western Blot, Control, Clinical Proteomics

Journal: The Journal of Clinical Investigation

Article Title: Absence of the intracellular lipolytic inhibitor G0S2 enhances intravascular triglyceride clearance and abolishes diet-induced hypertriglyceridemia

doi: 10.1172/JCI181754

Figure Lengend Snippet: ( A ) Time course of plasma TG levels during OLTT ( n = 10). ( B ) iAUC analysis of OLTT plasma TG curve ( n = 10). ( C ) Hepatic TG and cholesterol content ( n = 10). ( D ) Representative images of liver sections with H&E staining. Scale bars: 100 μm. ( E ) Hepatic TG secretion assay after Poloxamer 407 administration ( n = 6). ( F ) Fecal TG content ( n = 10). ( G ) Circulating LPL activity 10 minutes after PBS or heparin administration, as determined following a 15-minute reaction of plasma with an artificial substrate (detailed in Methods). Data represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 by unpaired 2-tailed t tests ( A , B , E , and F ) or 2-way ANOVA ( C and G ).

Article Snippet: Plasma cholesterol levels were measured using the Amplex Red Cholesterol Assay Kit (Thermo Fisher Scientific, A12216), according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Staining, Activity Assay

Journal: The Journal of Clinical Investigation

Article Title: Absence of the intracellular lipolytic inhibitor G0S2 enhances intravascular triglyceride clearance and abolishes diet-induced hypertriglyceridemia

doi: 10.1172/JCI181754

Figure Lengend Snippet: ( A ) Experimental scheme: After 3 weeks of treatment with Western diet (WD) and Ldlr-ASO, epididymal WAT dissected from chow-fed WT or G0s2 –/– donor mice was transplanted in situ into WT recipient mice. ( B ) Plasma TG concentrations prior to and after transplantation at the indicated time points ( n = 9–10). ( C and D ) Circulating lipase activity 10 minutes after heparin administration, as determined following a 15-minute reaction of plasma with the artificial substrate ( C ), and LPL protein concentration ( D ) during fasting or refeeding 4 weeks after transplantation ( n = 9–10). ( E – G ) Fasting plasma levels or free FA ( E ), total cholesterol ( F ), and glucose ( G ) at the end point ( n = 9–10). ( H ) Liver TG content normalized to liver weight at the end point ( n = 9–10). ( I ). Fasting plasma β-hydroxybutyrate (BHB) levels ( n = 9–10). Data represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 by unpaired 2-tailed t tests ( B , and E – I ) or 2-way ANOVA ( C and D ).

Article Snippet: Plasma cholesterol levels were measured using the Amplex Red Cholesterol Assay Kit (Thermo Fisher Scientific, A12216), according to the manufacturer’s instructions.

Techniques: Western Blot, In Situ, Clinical Proteomics, Transplantation Assay, Activity Assay, Protein Concentration